1. Remove strip from the pouch for immediate use.
2. Completely immerse reagent areas of the strip in fresh urine. Remove the strip immediately to avoid dissolving out the reagent areas.
3. While removing, touch the side of the strip against the rim of the urine container to remove excess urine. Blot the lengthwise edge of the strip on an absorbent paper towel to further remove excess urine and avoid running over (contamination from adjacent reagent pads.)
4. Compare each reagent area to its corresponding color blocks on the color chart and read at the times specified. Proper read time is critical for optimal results.
5. Obtain results by direct color chart comparison.
ASCORBIC ACID：Intend to measure the level of ascorbic acid(Vitamin C) in urine. Ascorbic Acid in higherquantities may cause interferences especially with the glucose test. The detections is based on the the decolorationof Tillmans reagent.
GLUCOSE: The test is specific for glucose. No substance excreted in urine other than glucose is known to give apositive result. In dilute urines containing less than 0.28 mmol/L ascorbic acid, as little as 2.2mmol/L glucose mayproduce a color change that might be interpreted as positive.
MICROALBUMIN: Testing albumin in urine is one of urine routine tests in clinic. This test is based on dye bindingusing a high affinity sulfonephthalein dye. At a constant pH, the development of any blue color is due to thepresence of albumin. As little as 80 mg/L albumin in urine may produce a color change. The resulting color rangesfrom pale green to blue.
BILIRUBIN: Normally no bilirubin is detectable in urine by even the most sensitive methods. Even trace amounts ofbilirubin are sufficiently abnormal to require further investigation. Atypical colors may indicate bile pigmentabnormalities and the urine specimens should be tested further(e.g. ICTOTESTTM Reagent Tablets).
pH: The pH test area measures pH values generally to within 1 unit in the range of 5-8.5 visually and 5-9instrumentally.
SPECIFIC GRAVITY: The specific gravity test permits determination of urine specific gravity between 1.000 and1.030. In general, it correlates within 0.005 with values obtained with the refractive index method.
KETONE: The test reacts with acetoacetic acid in urine. It does not react with acetone or B-hydroxbutyric acid.Some high specific gravity or low pH urines may give reactions up to and including Trace. Normal urine specimensusually yield negative results with this reagent. False positive results (trace or less) may occur with highlypigmented urine specimens or those containing large amounts or levodopa metabolites.
PROTEIN: The reagent area is more sensitive to albumin than to globulins, haemoglobin, Bence-Jones Protein andmucoprotein, a 'Negative' result does not rule out the presence of these other protein. Normally no protein isdetectable in urine by conventional methods, although a minute amount is excreted by the normal kidney.
NITRITE: This test depends upon the conversion of nitrate (derived from the diet) to nitrite by the action ofprincipally Gram negative bacteria in the urine. The test is specific for nitrite and will not react with any othersubstance normally excreted in urine. Pink spots or pink edges should not be interpreted as a positive result. Any degree of uniform pink color development should be interpreted as a positive nitrite test suggesting the presence of10 or more organisms per mL, but color development is not proportional to the number of bacteria present.
UROBILINOGEN: This test area will detect urobilinogen in concentrations as low as 3 μmol/L (approximately 0.2Ehrlich unit/dL) in urine. The normal range with this test is 3-16 μmol/L. A result of 33 μmol/L represents thetransition from normal to abnormal, and the patient and/or urine specimen should be evaluated further.
CREATININE: In alkaline condition, creatinine reacts with 3,5-Dinitrobenoic acid. The resulting color ranges frompale yellow to purple.
BLOOD: The significance of the 'Trace' reaction may vary among patients, and clinical judgment is required forassessment in an individual case. Development of green spots (intact erythrocytes) or green color (freehaemoglobin / myoglobin) on the reagent area within 60 seconds indicates the need for further investigation.
CALCIUM: The calcium in urine reacts with derivatized thymol phthalein. The resulting color ranges from pale purple to dark purple.
SPECIFIC PERFORMANCE CHARACTERISTICS: Specific performance characteristics are based on clinical andanalytical studies. In clinical specimens, the sensitivity depends upon several factors: the variability of colorperception, the presence or absence of inhibitory. Factors typically found in urine, specific gravity, pH and thelighting conditions when the product is read visually.
